abstract：AIM:To develop in vitro assays for comparing the antagonistic properties and anti-oxidative activity of probiotic Lactobacillus and Bifidobacterium strains against various entero- and urinary pathogens. METHODS AND RESULTS:The antagonistic activity of five probiotic lactobacilli (Lactobacillus rhamnosus GG, Lactobacil...

journal_title：Journal of applied microbiology

authors： Hütt P,Shchepetova J,Lõivukene K,Kullisaar T,Mikelsaar M

更新日期：2006-06-01 00:00:00

abstract：AIMS:To generate continuous minimum inhibitory concentration (MIC) data that describes the discrete nature of experimentally derived population MIC data. METHODS AND RESULTS:A logistic model was fitted to experimentally derived MIC population cumulative distributions from clinical isolates of Haemophilus influenzae, M...

journal_title：Journal of applied microbiology

authors： Lambert RJ,Lambert R

更新日期：2006-05-01 00:00:00

abstract：AIMS:To develop a fast and sensitive protocol for detection of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine semen and to make a critical evaluation of the analytical sensitivity. METHODS AND RESULTS:Processed semen was spiked with known amounts of MAP. Semen from different bulls as well as semen of diff...

journal_title：Journal of applied microbiology

authors： Herthnek D,Englund S,Willemsen PT,Bölske G

更新日期：2006-05-01 00:00:00

abstract：AIM:To quantify microbial aerosols generated by a series of laboratory accidents and to use these data in risk assessment. METHODS AND RESULTS:A series of laboratory accident scenarios have been devised and the microbial aerosol generated by them has been measured using a range of microbial air samplers. The accident ...

journal_title：Journal of applied microbiology

authors： Bennett A,Parks S

更新日期：2006-04-01 00:00:00

abstract：AIMS:To determine whether dosing with bacteriocin-producing Streptococcus salivarius following an antimicrobial mouthwash effects a change in oral malodour parameters and in the composition of the oral microbiota of subjects with halitosis. MATERIALS AND RESULTS:Twenty-three subjects with halitosis undertook a 3-day r...

journal_title：Journal of applied microbiology

authors： Burton JP,Chilcott CN,Moore CJ,Speiser G,Tagg JR

更新日期：2006-04-01 00:00:00

abstract：AIMS:To determine which intestinal section of pre and postweaned piglets are colonized by Lactobacillus plantarum 423 and Lactobacillus salivarius 241, and follow production of plantaricin 423 in a gastro-intestinal model. METHODS AND RESULTS:Lactobacillus plantarum 423 and Lact. salivarius 241, single or in combinati...

journal_title：Journal of applied microbiology

authors： Maré L,Wolfaardt GM,Dicks LM

更新日期：2006-04-01 00:00:00

abstract：AIMS:To evaluate beneficial effect of two food additives, ammonium molybdate (NH4-Mo) and sodium bicarbonate (NaBi), on antagonistic yeasts for control of brown rot caused by Monilinia fructicola in sweet cherry fruit under various storage conditions. The mechanisms of action by which food additives enhance the efficac...

journal_title：Journal of applied microbiology

authors： Qin GZ,Tian SP,Xu Y,Chan ZL,Li BQ

更新日期：2006-03-01 00:00:00

abstract：AIMS:Gordonia jacobaea is a recently isolated bacterial species with potential industrial application on account of its ability to store large quantities of trans-canthaxanthin. Its genetic manipulation is, however, difficult and cumbersome owing to the presence of mycolic acids in the cell wall and, especially, becaus...

journal_title：Journal of applied microbiology

authors： Veiga-Crespo P,Feijoo-Siota L,de Miguel T,Poza M,Villa TG

更新日期：2006-03-01 00:00:00

abstract：AIM:To evaluate the effect of NaCl, growth medium and temperature on the antimicrobial activity of bovine lactoferrin (LF) against Escherichia coli O157:H7 in the presence of different chelating agents. METHODS AND RESULTS:LF (32 mg ml(-1)) was tested against E. coli O157:H7 strain 3081 in Luria broth (LB) and All Pur...

journal_title：Journal of applied microbiology

authors： Al-Nabulsi AA,Holley RA

更新日期：2006-02-01 00:00:00

abstract：AIM:To investigate the influence of several environmental factors on the viability and cell-adsorption for two Lactobacillus casei/paracasei bacteriophages (PL-1 and J-1). METHODS AND RESULTS:Both phages showed a remarkably high specificity of species, sharing similar host spectra. Two phages and four sensitive strain...

journal_title：Journal of applied microbiology

authors： Capra ML,Quiberoni A,Reinheimer J

更新日期：2006-02-01 00:00:00

abstract：AIMS:To determine the incidence of emetic toxin producing Bacillus cereus in soil, animal faeces and selected vegetable produce to compare the results with the previously reported high incidence in rice paddy fields. To examine whether the emetic toxin has antibiotic activity. METHODS AND RESULTS:The incidence of emet...

journal_title：Journal of applied microbiology

authors： Altayar M,Sutherland AD

更新日期：2006-01-01 00:00:00

abstract：AIMS:To describe the occurrence and virulence gene pattern of shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) in healthy goats of Jammu and Kashmir, India. METHODS AND RESULTS:A total of 220 E. coli strains belonging to 60 different 'O' serogroups was isolated from 206 local (nonmigra...

journal_title：Journal of applied microbiology

authors： Wani SA,Samanta I,Munshi ZH,Bhat MA,Nishikawa Y

更新日期：2006-01-01 00:00:00

abstract：AIMS:Fungal infection is still a life-threatening risk for the immunocompromised population such as AIDS patients and those who receive treatments with immunosuppressors and/or frequent administrations of wide-spectrum antibiotics, which inevitably lead to the drug resistance and unbalanced microflora populations. The ...

journal_title：Journal of applied microbiology

authors： Liu JY,Huang LL,Ye YH,Zou WX,Guo ZJ,Tan RX

更新日期：2006-01-01 00:00:00

abstract：AIMS:To investigate the sort and the origin of the contamination of a packed fruit juice. METHODS AND RESULTS:Fifty-eight yeast isolates were collected in a survey of two different visits to a carbonated orange juice factory. In each visit, samples were collected, six times, from seven points in the production chain. ...

journal_title：Journal of applied microbiology

authors： Pina C,Teixeiró P,Leite P,Villa M,Belloch C,Brito L

更新日期：2005-01-01 00:00:00

abstract：AIMS:The aim of this work was to measure the dynamic global metabolic activities of the microbial community during ripening of RDO Salers cheese by using a direct molecular approach. METHODS AND RESULTS:A culture-independent approach including PCR, reverse transcriptase PCR (RT-PCR) and single strand conformation poly...

journal_title：Journal of applied microbiology

authors： Duthoit F,Tessier L,Montel MC

更新日期：2005-01-01 00:00:00

abstract：AIMS:The aim of the present study is to identify genes and proteins whose expression is induced in lager brewing yeast during the lag phase and early exponential growth. METHODS AND RESULTS:Two-dimensional gel electrophoresis was used to identify proteins induced during the lag and early exponential phase of lager bre...

journal_title：Journal of applied microbiology

authors： Brejning J,Arneborg N,Jespersen L

更新日期：2005-01-01 00:00:00

abstract：AIMS:The objective of the study was to compare ultraviolet (u.v.) inactivation kinetics of indigenous aerobic spores in surface water with their laboratory-cultured spore isolates and to investigate the relationship between physicochemical characteristics and u.v. inactivation kinetics of spore isolates. METHODS AND R...

journal_title：Journal of applied microbiology

authors： Mamane-Gravetz H,Linden KG

更新日期：2005-01-01 00:00:00

abstract：AIMS:To characterize the bioemulsifier produced by a nonfluorescent strain of Pseudomonas putida isolated from a polluted sediment and to determine the influence of pH, temperature, media composition, and carbon and nitrogen source on growth and emulsifying activity. METHODS AND RESULTS:Different indexes were employed...

journal_title：Journal of applied microbiology

authors： Bonilla M,Olivaro C,Corona M,Vazquez A,Soubes M

更新日期：2005-01-01 00:00:00

abstract：AIMS:To identify native Antarctic bacteria capable of oil degradation at low temperatures. METHODS AND RESULTS:Oil contaminated and pristine soils from Signy Island (South Orkney Islands, Antarctica) were examined for bacteria capable of oil degradation at low temperatures. Of the 300 isolates cultured, Pseudomonas st...

journal_title：Journal of applied microbiology

authors： Stallwood B,Shears J,Williams PA,Hughes KA

更新日期：2005-01-01 00:00:00

abstract：AIM:To determine the effect of different carbohydrate-based finishing diets on fermentation characteristics and the shedding of Escherichia coli and enterohaemorrhagic E. coli (EHEC) virulence genes in cattle faeces. METHODS AND RESULTS:The size of faecal E. coli populations and fermentation characteristics were ascer...

journal_title：Journal of applied microbiology

authors： Gilbert RA,Tomkins N,Padmanabha J,Gough JM,Krause DO,McSweeney CS

更新日期：2005-01-01 00:00:00

abstract：AIMS:Salmonella Hadar, Salmonella Brancaster and Salmonella Enteritidis are the main Salmonella enterica ssp. enterica serovars isolated from poultry in Senegal. Our objective was to analyse the pulsed-field gel electrophoresis (PFGE) and antibioresistance patterns of strains belonging to these serovars and to assess t...

journal_title：Journal of applied microbiology

authors： Cardinale E,Perrier Gros-Claude JD,Rivoal K,Rose V,Tall F,Mead GC,Salvat G

更新日期：2005-01-01 00:00:00

abstract：AIMS:To study the potential apoptosis effects of cytotoxic marine bacterial metabolites on human HeLa cell line. METHODS AND RESULTS:After HeLa cells were routinely cultured, tetrazolium-based colorimetric assay for cytotoxicity was performed to screen the marine bacteria extracts showing 12 strains active. To find th...

journal_title：Journal of applied microbiology

authors： Lin J,Yan XJ,Zheng L,Ma HH,Chen HM

更新日期：2005-01-01 00:00:00

abstract：AIMS:Plasmid profile, phage typing, and pulsed-field gel electrophoresis (PFGE) patterns of 124 Salmonella Enteritidis strains isolated in 1998-2002 in Taiwan were analysed and the results were compared with those of the 63 strains obtained in 1991-1997, so that molecular subtypes and epidemic strains for Salmonella En...

journal_title：Journal of applied microbiology

authors： Pang JC,Chiu TH,Chiou CS,Schroeter A,Guerra B,Helmuth R,Tsen HY

更新日期：2005-01-01 00:00:00

abstract：AIMS:To develop a real-time (rt) PCR for species differentiation of thermophilic Campylobacter and to develop a method for assessing co-colonization of pigs by Campylobacter spp. METHODS AND RESULTS:The specificity of a developed 5' nuclease rt-PCR for species-specific identification of Campylobacter jejuni, Campyloba...

journal_title：Journal of applied microbiology

authors： Jensen AN,Andersen MT,Dalsgaard A,Baggesen DL,Nielsen EM

更新日期：2005-01-01 00:00:00

abstract：AIM:The main aim of the present study was to use three PCR-based techniques for the analysis of genetic variability among Vibrio parahaemolyticus strains isolated from the Philippines. METHODS AND RESULTS:Seventeen strains of V. parahaemolyticus isolated from shrimps (Penaeus monodon) and from the environments where t...

journal_title：Journal of applied microbiology

authors： Maluping RP,Ravelo C,Lavilla-Pitogo CR,Krovacek K,Romalde JL

更新日期：2005-01-01 00:00:00

abstract：AIMS:To study the modification of the cell wall of Lactobacillus casei ATCC 393 grown in high salt conditions. METHODS AND RESULTS:Differences in the overall structure of cell wall between growth in high salt (MRS + 1 mol l(-1) NaCl; N condition) and control (MRS; C condition) conditions were determined by transmissio...

journal_title：Journal of applied microbiology

authors： Piuri M,Sanchez-Rivas C,Ruzal SM

更新日期：2005-01-01 00:00:00

abstract：AIMS:This study elucidates the mechanisms by which a nonbacteriocinogenic Carnobacterium piscicola inhibits growth of Listeria monocytogenes. METHODS AND RESULTS:Listeria monocytogenes was exposed to live cultures of a bacteriocin-negative variant of C. piscicola A9b in co-culture, in a diffusion chamber system, and t...

journal_title：Journal of applied microbiology

authors： Nilsson L,Hansen TB,Garrido P,Buchrieser C,Glaser P,Knøchel S,Gram L,Gravesen A

更新日期：2005-01-01 00:00:00

abstract：AIMS:The aim of this study was to investigate the influence of environmental and physiological factors on the susceptibility of Escherichia coli to the Curvularia haloperoxidase system. METHODS AND RESULTS:The Curvularia haloperoxidase system is a novel enzyme system that produces reactive oxygen species which have an...

journal_title：Journal of applied microbiology

authors： Hansen EH,Schäfer T,Molin S,Gram L

更新日期：2005-01-01 00:00:00

abstract：AIMS:Forty Bacillus strains isolated from a Brazilian oil reservoir were tested against each other to select strains producing antimicrobial substances (AMS). Three strains, Bacillus subtilis (LFE-1), Bacillus firmus (H2O-1) and Bacillus licheniformis (T6-5), were selected due to their ability to inhibit more than 65% ...

journal_title：Journal of applied microbiology

authors： Korenblum E,der Weid I,Santos AL,Rosado AS,Sebastián GV,Coutinho CM,Magalhães FC,Paiva MM,Seldin L

更新日期：2005-01-01 00:00:00

abstract：AIMS:Bacillus licheniformis PWD-1 is a keratin-degrading, spore-forming bacterium isolated from a poultry waste digester. A sporulation-deficient mutant of B. licheniformis PWD-1, named B. licheniformis WBG, was developed and characterized. METHODS AND RESULTS:The mutation was generated using the splicing by overlap e...

journal_title：Journal of applied microbiology

authors： Wang JJ,Greenhut WB,Shih JC

更新日期：2005-01-01 00:00:00

abstract：AIMS:To investigate the occurrence and extent of Saccharomyces cerevisiae and Oenococcus oeni interactions. METHODS AND RESULTS:Interactions between S. cerevisiae and O. oeni were investigated by double-layer and well-plate assays showing the occurrence of specific interactions for each yeast-malolactic bacteria (MLB)...

journal_title：Journal of applied microbiology

authors： Comitini F,Ferretti R,Clementi F,Mannazzu I,Ciani M

更新日期：2005-01-01 00:00:00

abstract：AIM:To assess the role of lactate as a precursor for butyrate biosynthesis in human colonic microflora. METHODS AND RESULTS:Three human faecal microfloras were incubated in vitro with media supplemented with 30 mmol l(-1) unenriched or 13C-enriched lactate. Lactate metabolism and short-chain fatty acid (SCFA) producti...

journal_title：Journal of applied microbiology

authors： Bourriaud C,Robins RJ,Martin L,Kozlowski F,Tenailleau E,Cherbut C,Michel C

更新日期：2005-01-01 00:00:00

abstract：AIMS:This study examined the tolerance of various species of the genus Bifidobacterium to heat and oxygen and evaluated the survival of selected strains following spray drying and during storage. METHODS AND RESULTS:Nine Bifidobacterium species were considered to be relatively tolerant to both heat and oxygen and most...

journal_title：Journal of applied microbiology

authors： Simpson PJ,Stanton C,Fitzgerald GF,Ross RP

更新日期：2005-01-01 00:00:00

abstract：AIMS:To quantify the ability of 136 lactic acid bacteria (LAB), isolated from wine, to produce histamine and to identify the bacteria responsible for histamine production in wine. METHODS AND RESULTS:A qualitative method based on pH changes in a plate assay was used to detect wine strains capable of producing high lev...

journal_title：Journal of applied microbiology

authors： Landete JM,Ferrer S,Pardo I

更新日期：2005-01-01 00:00:00

abstract：AIMS:The effectiveness of the food-grade antioxidants butylated hydroxytoluene (BHT), trihydroxybutyrophenone (THB), propyl paraben (PP) and butylated hydroxyanisole (BHA) at 1, 10 and 20 mmol l(-1) concentrations on germination, growth, and aflatoxin B(1) (AFB(1)) production by Aspergillus section Flavi strains was de...

journal_title：Journal of applied microbiology

authors： Passone MA,Resnik SL,Etcheverry MG

更新日期：2005-01-01 00:00:00

abstract：AIMS:The dynamics of bioaerosol generation in specific occupational environments where mail is manually unpacked and sorted was investigated. METHODS AND RESULTS:Total number of airborne particles was determined in four different size classes (0.3-0.5, 0.5-1, 1-5 and >5 microm) by laser particle counting. Time depende...

journal_title：Journal of applied microbiology

authors： Brandl H,Bachofen R,Bischoff M

更新日期：2005-01-01 00:00:00

abstract：AIMS:Three broadly used typing methods were employed in order to assess and compare the identification and classification of environmental Pseudomonas strains. The reproducibility, typeability and discriminatory power of the methods were also compared to evaluate their application. Finally, the potential impact on publ...

journal_title：Journal of applied microbiology

authors： Sazakli E,Leotsinidis M,Vantarakis A,Papapetropoulou M

更新日期：2005-01-01 00:00:00

abstract：AIMS:The biopesticide effect of four green composts against fusarium wilt in melon plants and the effect of soil quality in soils amended with composts were assayed. METHODS AND RESULTS:The composts consisted of pruning wastes, with or without addition of coffee wastes (3/1 and 4/1, dry wt/dry wt) or urea (1000/1, dry...

journal_title：Journal of applied microbiology

authors： Ros M,Hernandez MT,Garcia C,Bernal A,Pascual JA

更新日期：2005-01-01 00:00:00

abstract：AIM:Evaluation of the Escherichia coli genome for variable number tandem repeat (VNTR) loci in order to provide a subtyping tool with greater discrimination and more efficient capacity. METHODS AND RESULTS:Twenty-nine putative VNTR loci were identified from the E. coli genomic sequence. Their variability was validated...

journal_title：Journal of applied microbiology

authors： Keys C,Kemper S,Keim P

更新日期：2005-01-01 00:00:00

abstract：AIMS:To evaluate an integrated cell culture (ICC)-multiplex-nested PCR using the buffalo green monkey kidney (BGMK) cells for the simultaneous detection of both enteroviruses and adenoviruses in surface water and tap water samples and optimize the procedure for more sensitive detection of virus showing no apparent cyto...

journal_title：Journal of applied microbiology

authors： Lee SH,Lee C,Lee KW,Cho HB,Kim SJ

更新日期：2005-01-01 00:00:00